Data normalization considerations for digital tumor dissection

cDNA Microarray Data Normalization

Considerations for normalization of DNA methylation data by Illumina 450K BeadChip assay in population studies.

Analysis of epigenetic mechanisms, particularly DNA methylation, is of increasing interest for epidemiologic studies examining disease etiology and impacts of environmental exposures. The Infinium HumanMethylation450 BeadChip(®) (450K), which interrogates over 480,000 CpG sites and is relatively cost effective, has become a popular tool to characterize the DNA methylome. For large-scale studies...

Group Normalization for Genomic Data

Data normalization is a crucial preliminary step in analyzing genomic datasets. The goal of normalization is to remove global variation to make readings across different experiments comparable. In addition, most genomic loci have non-uniform sensitivity to any given assay because of variation in local sequence properties. In microarray experiments, this non-uniform sensitivity is due to differe...

Legal Considerations in the Dissemination of Licensed Digital Spatial Data

State and local governments increasingly license digital spatial data, the dissemination of which by academic libraries requires specifi c legal and operational considerations to reconcile license conditions with public access. We examined this in the context of the American Geographical Society Library (AGSL) at the University of Wisconsin–Milwaukee during 2000-05. Wisconsin open records law p...

Systems Linearity Considerations for Digital Photography

End-to-end system studies in digital photography inevitably lead to the question of setting goals in order to achieve overall linearity. But this itself poses the specific question of which key print/display variables should, in ideal circumstances, be linear with which key sceneacquisition parameters. Within the digital context, intuitive answers gleaned from traditional analog approaches to t...